Miniature CRISPR Tool for Plant Genome Editing

Researchers have developed a miniature CRISPR tool, TnpB, for plant genome editing, addressing the challenges posed by the large size of traditional CRISPR proteins like Cas9 and Cas12. Derived from the bacterium Deinococcus radiodurans, TnpB is more effective due to its smaller size and precise editing capabilities, with a success rate of 33.58% in plants. Alongside TnpB, a hybrid base editor was created to expand editing possibilities. Although early results are promising, further research is needed to confirm the tool’s effectiveness in different crops, potentially leading to stronger, more nutritious plants capable of adapting to climate change.

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